FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n02_5P-R_cDNA-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n02_5P-R_cDNA-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40179042
Sequences flagged as poor quality0
Sequence length49
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT5701291.418971114343642No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1231670.30654538751819915No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT948790.23614052321108106No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT432390.10761580627034362No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT736050.036.3972361
AGCAGTG810700.033.056782
ATCAACG861800.030.90428211
TCAACGC871850.030.58036612
TGGTATC877650.030.4368697
CAACGCA879700.030.32489413
GTATCAA884150.030.1464129
TATCAAC888450.030.01547410
AACGCAG897350.029.8077314
GGTATCA897000.029.77838
ACGCAGA906150.029.4828115
AGTGGTA915150.029.2706495
AGAGTAC909650.029.23881519
GTGGTAT915000.029.2192426
CAGTGGT919500.029.1598344
CGCAGAG933500.028.74890516
GAGTACT929750.028.41917820
GCAGAGT939800.028.41441317
CAGAGTA947950.028.17542618
GTACTTT945950.027.84527822
Produced by FastQC (version 0.11.5)