FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n02_5P-R_cDNA-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n02_5P-R_cDNA-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62279944
Sequences flagged as poor quality0
Sequence length49
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT8582711.378085696416169No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1883110.30236218581057167No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1449340.23271376095007407No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT656320.10538223990695944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1107450.036.2438051
AGCAGTG1222200.032.8336372
ATCAACG1294000.030.8026111
TCAACGC1310300.030.41972512
TGGTATC1319800.030.2125137
CAACGCA1325400.030.09773613
GTATCAA1331100.029.9692659
TATCAAC1339400.029.7951210
AACGCAG1351850.029.5948314
GGTATCA1348950.029.5761688
ACGCAGA1366600.029.24704215
GTGGTAT1381350.028.930636
CAGTGGT1384400.028.9203154
AGAGTAC1374950.028.9049519
AGTGGTA1385400.028.8873755
CGCAGAG1409850.028.42132416
GAGTACT1404100.028.13481920
GCAGAGT1421850.028.07903517
CAGAGTA1431100.027.928618
GTACTTT1434550.027.40696322
Produced by FastQC (version 0.11.5)