FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n02_5P-R_cDNA-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n02_5P-R_cDNA-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60323346
Sequences flagged as poor quality0
Sequence length49
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT8421291.3960250149253988No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1853470.30725583425030834No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1418860.235209101298857No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT638980.10592582182029492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1079100.036.418841
AGCAGTG1194900.032.8853842
ATCAACG1257100.031.03157211
TCAACGC1274800.030.61601612
TGGTATC1284900.030.4342257
CAACGCA1287000.030.35599313
GTATCAA1290750.030.2618529
TATCAAC1301300.030.03833810
AACGCAG1312500.029.81879214
GGTATCA1312400.029.8119438
ACGCAGA1327700.029.44183715
GTGGTAT1339800.029.239086
AGAGTAC1334800.029.19451719
AGTGGTA1347700.029.1115535
CAGTGGT1355200.029.0070464
CGCAGAG1365750.028.72058116
GAGTACT1365250.028.34326620
GCAGAGT1381500.028.3108517
CAGAGTA1388050.028.21705418
GTACTTT1391350.027.73076622
Produced by FastQC (version 0.11.5)