FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n02_5P-R_cDNA-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n02_5P-R_cDNA-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59802254
Sequences flagged as poor quality0
Sequence length49
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT8484701.4187926762760479No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1811870.3029768744168071No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT1417770.23707634832626878No Hit
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT642780.10748424298522259No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT1088850.036.580991
AGCAGTG1199450.033.224042
ATCAACG1276700.031.06810611
TCAACGC1291350.030.70416812
CAACGCA1303050.030.4420413
TGGTATC1304550.030.421057
GTATCAA1309950.030.306969
TATCAAC1322000.030.05215810
AACGCAG1330250.029.890914
GGTATCA1328900.029.8899428
ACGCAGA1344200.029.56956915
AGAGTAC1349700.029.36601819
AGTGGTA1360050.029.2431165
GTGGTAT1359300.029.2297616
CAGTGGT1364050.029.1885644
CGCAGAG1381650.028.83853316
GAGTACT1381050.028.54673620
GCAGAGT1398100.028.43020617
CAGAGTA1404400.028.31103518
GTACTTT1407100.027.90166922
Produced by FastQC (version 0.11.5)