FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n02_5P-G_cDNA-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n02_5P-G_cDNA-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22700109
Sequences flagged as poor quality0
Sequence length49
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT2372211.0450214137738283No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG562110.24762436162751467No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT365960.16121508491434997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT317400.035.2014851
ATCAACG354750.031.34102811
AGCAGTG359000.031.1504462
TCAACGC361250.030.75918412
CAACGCA363850.030.55153813
GTATCAA367700.030.3242769
TGGTATC368850.030.2860897
TATCAAC369700.030.13736210
AACGCAG374700.029.78203214
GGTATCA376300.029.6872658
ACGCAGA379800.029.34255215
AGAGTAC379250.029.24304619
GTGGTAT388050.028.7698886
AGTGGTA392250.028.4833815
CGCAGAG394050.028.34027516
CAGTGGT395050.028.3190334
GAGTACT394350.027.97045320
GCAGAGT397650.027.91615117
CAGAGTA400100.027.84798618
GTACTTT403750.027.24427622
Produced by FastQC (version 0.11.5)