FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n02_5P-G_cDNA-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n02_5P-G_cDNA-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32810268
Sequences flagged as poor quality0
Sequence length49
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT3431471.0458524752068468No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG778660.23732204808567856No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT523670.15960552349039026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT467100.035.0308041
AGCAGTG519850.031.4906982
ATCAACG514150.031.48512311
TCAACGC523450.030.92181612
CAACGCA528100.030.68225713
GTATCAA532150.030.4518579
TGGTATC535750.030.3060467
TATCAAC539550.030.06251110
AACGCAG541150.030.02993814
GGTATCA542250.029.9472318
ACGCAGA548950.029.5836615
AGAGTAC552250.029.29755419
GTGGTAT563850.028.8479546
AGTGGTA569400.028.6301735
CAGTGGT575100.028.3759674
GAGTACT568600.028.28112220
CGCAGAG576950.028.23210116
GCAGAGT580000.027.96518717
CAGAGTA580600.027.95212218
GTACTTT583850.027.4351722
Produced by FastQC (version 0.11.5)