FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n02_5P-G_cDNA-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n02_5P-G_cDNA-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43557910
Sequences flagged as poor quality0
Sequence length49
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT4430711.017199861058531No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1063780.24422200238716688No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT682310.1566443385369041No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT603350.034.8313141
ATCAACG671700.031.04964411
AGCAGTG676700.031.0381532
TCAACGC682800.030.5419112
CAACGCA690000.030.23581713
GTATCAA693300.030.0784919
TGGTATC695550.030.0350767
TATCAAC703350.029.69497110
AACGCAG706950.029.59924714
GGTATCA711950.029.3618988
ACGCAGA713950.029.27306715
AGAGTAC722000.028.87454819
GTGGTAT733000.028.5613486
AGTGGTA741300.028.2701075
CAGTGGT747550.028.1542784
CGCAGAG746950.028.1395116
GAGTACT746450.027.78752320
GCAGAGT755900.027.70412417
CAGAGTA760200.027.55389618
GTACTTT758950.027.17905622
Produced by FastQC (version 0.11.5)