FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n02_5P-G_cDNA-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n02_5P-G_cDNA-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34383810
Sequences flagged as poor quality0
Sequence length49
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTT3400430.9889625378921068No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG836370.2432452948058985No Hit
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT532650.1549130244728551No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGCAGT463300.034.947051
AGCAGTG518700.031.24592
ATCAACG516750.031.1933911
TCAACGC527100.030.57299412
CAACGCA531950.030.32293713
GTATCAA535850.030.1491649
TGGTATC536350.030.1236677
TATCAAC541650.029.80272710
AACGCAG546500.029.54722414
GGTATCA550400.029.3629848
ACGCAGA556000.029.06557315
AGAGTAC556400.028.94781519
GTGGTAT566850.028.5588476
AGTGGTA571850.028.3387225
CAGTGGT577850.028.0367094
CGCAGAG581650.027.87140516
GAGTACT575850.027.79437820
CAGAGTA587550.027.48986618
GCAGAGT587850.027.48259417
GTACTTT589150.027.03514722
Produced by FastQC (version 0.11.5)