FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30171810
Sequences flagged as poor quality0
Sequence length26
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGG1249010.41396588404871965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT38350.017.2798391
TACGGGC53900.016.951721
CTCCCTG46200.016.9232229
GCGCGAT24800.016.5994361
CGATTGA37700.016.459181
TCTTGAG23650.015.9799998
TGACTAG35050.015.9275111
CCGGGAT23250.015.8922721
GACCTGG28200.015.8798681
AGTGAAC27450.015.7710888
GGAACTT31550.015.6894861
GGGACCT20950.015.6720161
ACAGCCG38400.015.58391
TTTACTG29700.015.5511511
CTCGAAA30800.015.5499441
CGCTTCA31950.015.493061
GCTCTGT37150.015.486631
ACCCACT35050.015.4691661
CCGTACT38800.015.4491211
CCGGTAG27250.015.4017811
Produced by FastQC (version 0.11.5)