FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n01_5P_R-HTO.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n01_5P_R-HTO.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8857759
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGAT160650.019.1910118
CTGATTC144050.019.1603459
ACCGATA160950.019.1367079
ATAGTCT147300.019.1176684
CGGTACA166200.019.0193653
CCTATTA60050.018.9692461
TGATTCT145300.018.94044910
TAGTCTG145450.018.887185
CCGATAT163200.018.87900210
GCGGTAC167200.018.7680552
ACACCGA163350.018.7635027
ACATAGT146050.018.7471162
CTCGGGA100800.018.7219331
CGGGAAG87400.018.6957633
GGCTTGG76450.018.65303410
CGGCTTG76600.018.6295649
CATAGTC147050.018.6264273
GGGCTCT100050.018.5910249
TCGGGAA88050.018.5577492
TCTGATT149150.018.5453038
Produced by FastQC (version 0.11.5)