FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n01_5P_R-GDO_lenti.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n01_5P_R-GDO_lenti.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10994522
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCTTGA75900.019.092591
TCAGCAA158000.018.9953421
CAATGGA91100.018.9579948
AAGACCT81150.018.9295771
TAACCTG118650.018.9136610
AGCAACA109550.018.8404853
CCGCCAG89700.018.7961034
GCCGCCA90900.018.7786523
ATAGTGC98150.018.7779245
GCGCATA112050.018.7510995
ATAACCT119950.018.7420259
GACCTGT79850.018.7223783
TGCGCAT98950.018.686324
CACCGAA51250.018.6739648
TAGTGCT100100.018.6723776
TCGAATG83400.018.6218958
ACCCACT70250.018.6208571
TCTCATA129250.018.5935131
AACAGCT112300.018.5762886
TCATAGT114250.018.564333
Produced by FastQC (version 0.11.5)