FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n01_5P_R-GDO_CITE.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n01_5P_R-GDO_CITE.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14350473
Sequences flagged as poor quality0
Sequence length26
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCCA134150.019.0085853
AGCTTGA74500.018.7004761
CCGCCAG132800.018.6748434
ATGCGAT77700.018.6125161
CAATGGA80500.018.4475388
ATCACTA98100.018.3904366
GGCGACT63550.018.3187241
ACTGAAC57550.018.2995681
ATGATCA109100.018.285993
CCGTACT77200.018.137131
GCGCGAT48750.018.1357481
CTTGCCG70350.018.0814139
CACTAGT108800.018.0517988
CACACCT88050.018.0489651
TGACTAG65250.018.0407141
AATGGAA57800.017.9588669
GCATGAT206550.017.9302481
GGAAAGC69450.017.9289511
TCTTGCC71200.017.9216738
GCTCCGA99150.017.90255510