FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n01_5P_R-BLG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n01_5P_R-BLG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16478562
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGC1455700.019.8486231
CGGGCTC1464000.019.6134533
CTCCCTG1464500.019.5688159
CTCTCCC1463100.019.5000537
CCCTGAA239450.019.49515511
GGGTTCT617900.019.4887144
TCCCTGA1471200.019.45658710
CCCTGAT480900.019.42863311
CCCTGAG250900.019.41857311
TGGGTTC621400.019.3998073
GGGCTCT1472550.019.3991264
TCTCCCT1480100.019.3921178
GCTGGGT639700.019.3670961
CTGGGTT622300.019.3573472
GCTCTCC1477950.019.3107416
GGCTCTC1480200.019.2975435
ACGGGCT1493450.019.2341122
CCCTGAC513400.019.01098411
CCTGACC141500.018.86196712
CCTGATC169000.018.8459312