FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n01_5P_G-GDO_lenti.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n01_5P_G-GDO_lenti.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13362588
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGTTA231550.019.1415775
CTGATAG125100.019.1392691
TTCTCCT172400.019.138311
CCAGGTT231600.019.110824
AACGCGA200300.019.05177910
TTGTAAC177800.019.033766
GATGTTG178950.018.9886152
ACAGCCG95600.018.9251441
TTATCAC172950.018.9133349
CCTTCGA136200.018.908571
TATCACG173050.018.89084610
AGCTTGA117350.018.8436131
GAAGACC86250.018.8417076
CGAAGAC86200.018.806095
AGCTAAT73350.018.8003432
GACCCAC90150.018.7912569
ACAGTGG121100.018.7451910
TAACGCG203150.018.7155869
GTTGTAA180100.018.7072545
CTTCGAA88900.018.6839852