FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n01_5P_G-GDO_CITE.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n01_5P_G-GDO_CITE.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16232789
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT73000.018.1797051
TCTGGAA91350.017.9873751
CTGGAAA73600.017.9621222
CGATTGA76600.017.7561551
GGAATAA70250.017.7524741
CAATGGA61950.017.6917428
GGGAGAT55400.017.6550061
AGACGAA59650.017.5693710
TGGAAAG75200.017.5666523
TTGACTT75500.017.5644841
GAACCTA72150.017.5067621
GGAGCGA92800.017.5001079
GGAGCAA91250.017.4810121
TGACGGC55100.017.4607261
ATAGACC45650.017.416671
TGAATTG57600.017.3092410
GACGCGT62200.017.2362671
AAACCTG63650.017.1578561
GCATGCG79250.017.1119381
TGACTAG56650.017.0535961
Produced by FastQC (version 0.11.5)