FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n01_5P-R_cDNA-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n01_5P-R_cDNA-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40179042
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT137600.017.632551
ACTGATG135500.017.4408281
CAATGGA150250.017.1050828
CTCGAAA108600.016.999831
CGGACTG100200.016.8579441
AGTGAAC105500.016.6921928
TGCTACC121450.016.642231
TATGCCC116100.016.6252371
CTGCCTA132150.016.6191161
GACCAAT142650.016.6017041
AGCTTGA112700.016.5854821
CGCTTCA120900.016.5773141
TACGGAT121100.016.5664521
GATGCTA99400.016.5609861
AAACCTG125900.016.5544471
ACTGAAC96500.016.5404871
TGATTTC131600.016.5213831
GCGCGAT80150.016.5081751
TGACTAG117950.016.3983921
TTGACTT151100.016.3947321
Produced by FastQC (version 0.11.5)