FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n01_5P-R_cDNA-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n01_5P-R_cDNA-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62279944
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT214900.017.5168481
ACTGATG202900.017.424091
CAATGGA236300.017.1437598
CTCGAAA171850.017.0514321
GCGCGAT127250.016.8189431
AAACCTG190400.016.7768611
GACCAAT219850.016.758531
TGATTTC210500.016.699981
CTGCCTA201900.016.6782131
AGTGAAC161000.016.6710978
CGCTTCA191850.016.66571
CGGACTG160700.016.6600111
AGCTTGA180000.016.6571941
GATGCTA163600.016.6275521
TTGACTT238800.016.5803591
ATAGACC175300.016.5789281
ACTGAAC146550.016.528411
TATGCCC184300.016.5127491
TACGGAT190850.016.443851
TGACTAG177850.016.391831
Produced by FastQC (version 0.11.5)