FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n01_5P-R_cDNA-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n01_5P-R_cDNA-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60323346
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATG198850.017.517531
ATGCGAT203250.017.4679831
CGGACTG154150.017.030631
CAATGGA227800.017.0195968
AAACCTG184400.016.9214921
CTGCCTA202900.016.8277661
CTCGAAA162000.016.7671681
GACCAAT211850.016.7069451
ACTGAAC140800.016.6565531
GATGCTA160750.016.6238041
TGATTTC199800.016.5883051
TACGGAT178800.016.567711
AGTGAAC161150.016.5190458
TTGACTT230450.016.4911841
CGCTTCA186900.016.4864581
GTGATAA148150.016.4565668
TGCTACC187400.016.4317971
GCGCGAT121450.016.3788051
GGAACTT178350.016.3627831
ATAGACC169800.016.338561
Produced by FastQC (version 0.11.5)