FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n01_5P-R_cDNA-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n01_5P-R_cDNA-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59802254
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCGAT209250.017.526061
ACTGATG201250.017.5021881
CAATGGA229850.016.9722798
CTCGAAA158600.016.956121
CGGACTG152650.016.8701631
ACTGAAC143850.016.72031
TACGGAT180200.016.610811
AGTGAAC151250.016.588768
GACCAAT208900.016.5787931
AAACCTG182250.016.5776161
CGCTTCA181950.016.5609761
TATGCCC171150.016.5600571
TTGACTT221350.016.5544531
TGATTTC203350.016.4952071
GCGCGAT115950.016.4654671
CTGCCTA200500.016.4204221
GATGCTA151900.016.4004151
AGCTTGA171550.016.3815291
TGACTAG167350.016.3623851
GTGATAA151150.016.341718
Produced by FastQC (version 0.11.5)