FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n01_5P-G_cDNA-4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n01_5P-G_cDNA-4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22700109
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA92600.017.3663751
TGACGGC73600.017.2024361
ATAGACC71700.016.9469381
GGATTAC75150.016.914171
ATGCGAT54400.016.8763261
GGAATAA90700.016.8149911
TACGGAT72850.016.7343271
ATAACGC75300.016.6945381
CGACCTT74800.016.6189521
GCGCGAT44950.016.5975231
GACTAAC55600.016.5840361
TATTGAG54400.016.5807233
TATGCCC70100.016.5776211
GGTATTG80600.016.5769841
ACTGAAC59850.016.5593621
CTCGAAA55350.016.5324631
CCTTACG60900.016.5201821
AGCTACC57450.016.518998
GATGCTA50150.016.5117651
AAACCTG74800.016.445141
Produced by FastQC (version 0.11.5)