FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n01_5P-G_cDNA-3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n01_5P-G_cDNA-3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32810268
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA127800.017.7092841
TGACGGC107150.017.2860641
AAACCTG108850.017.1539131
ACTGAAC81650.017.0134891
ATAACGC110200.016.9346921
TATTGAG77800.016.7611163
GGATTAC105050.016.7367121
ATGCGAT83200.016.7205721
TACGGAT106000.016.699931
ATAGACC106600.016.6997551
GGTATTG114200.016.6130161
GCGCGAT62950.016.6022831
ACTGATG87450.016.5941471
TGACTAG91100.016.500151
CGGACTG74950.016.492791
CGACCTT103300.016.4683951
TCTATTG127350.016.4446641
CTACATT140450.016.4418051
CGTGAGC94300.016.4068761
GGAATAA131800.016.4054281
Produced by FastQC (version 0.11.5)