FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n01_5P-G_cDNA-2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n01_5P-G_cDNA-2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43557910
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA178500.017.3294541
ATGCGAT112550.017.0074651
TGACGGC144250.016.895961
TATGCCC135750.016.8930231
AAACCTG137750.016.887341
GGATTAC142450.016.8005471
ACTGAAC106600.016.784031
ATAACGC149500.016.730761
ACTGATG117650.016.7292461
TACGGAT143350.016.6811161
GACTAAC107200.016.6714321
CGGACTG99800.016.6048561
ATAGACC135250.016.593151
GGAATAA166000.016.4956171
GCGCGAT88350.016.4928681
TATTGAG100250.016.4689523
AGAAACC131650.016.4225528
GGTATTG152100.016.3987371
TCTATTG168850.016.3830341
CGACCTT135200.016.3625741
Produced by FastQC (version 0.11.5)