FastQCFastQC Report
Sat 9 Mar 2019
H33HLBGXB_n01_5P-G_cDNA-1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33HLBGXB_n01_5P-G_cDNA-1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34383810
Sequences flagged as poor quality0
Sequence length26
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC112900.017.4597871
CGATTGA136800.017.3702721
ATAGACC106700.016.9933761
GGATTAC111650.016.9834461
AAACCTG114550.016.8503321
ACTGATG91350.016.8381351
ACTGAAC88100.016.7895281
ATAACGC116500.016.7399791
ATGCGAT87100.016.7067151
AGCTACC75300.016.6934268
GACTAAC88550.016.6929111
TACGGAT112600.016.6891651
TATGCCC102800.016.66521
TATTGAG76750.016.6515183
GGAATAA127600.016.6240271
GGTATTG122550.016.5174661
CGTGAGC100450.016.5174371
TGACTAG96400.016.4332851
CGACCTT111800.016.4239311
CTCTACG115850.016.4195291
Produced by FastQC (version 0.11.5)