FastQCFastQC Report
Tue 16 May 2023
H33GJBGXT_n01_R23h7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33GJBGXT_n01_R23h7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32132596
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC440928613.722159267803946No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA434761013.53021710415181No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA432672713.465227023674029No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC358202411.147633387604289No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT357462611.1246100377324No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC31522379.810091285497132No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT30977429.640497145017477No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGATACACCGAACCCCTAC5548631.726791697751405No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA3571661.1115379535472327No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGGT3422761.0651987159705365No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAATCACCGAACCCCTACC1908640.5939887334344228No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA1706650.5311273325068413No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAATCACCGATCCCCTACC1486330.46256144383727976No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGATCCCCTACC1386620.43153064881530273No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC983190.30597901271344524No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC981100.3053285828508845No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTGGT500090.1556332392191406No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCGCTACCAAC347900.10827011922721712No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGATCCCCTACC342540.10660203115864027No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)