FastQCFastQC Report
Tue 16 May 2023
H33GJBGXT_n01_R23_h7rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33GJBGXT_n01_R23_h7rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA3514.227642276422763No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC3213.008130081300814No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC3012.195121951219512No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA228.94308943089431No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC218.536585365853659No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT208.130081300813007No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT187.317073170731707No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT156.097560975609756No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG83.2520325203252036No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGATACACCGAACCCCTAC62.4390243902439024No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG52.0325203252032518No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGGT31.2195121951219512No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC20.8130081300813009No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGATCCCCTACC20.8130081300813009No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA20.8130081300813009No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTTA20.8130081300813009No Hit
ACGATGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACC10.40650406504065045No Hit
GATCGATAACGCATTTCTTGGGGAAGGGCGGAAACACGGAACCCGAACAA10.40650406504065045No Hit
CGATCATGATCGTCTTGTGGAAAGGACAAAACACCGAACCCCTACCAACT10.40650406504065045No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGANNNCCNNCCAANNNGT10.40650406504065045No Hit
CGATCATGATCGTCTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG10.40650406504065045No Hit
GATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA10.40650406504065045No Hit
ATCGATTCCTTGGTTCTTGTGGATAGGACGAAACACCGAACCCCTACCAA10.40650406504065045No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCTAACCCCTACCAACTG10.40650406504065045No Hit
ACGATCGATAGGTATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC10.40650406504065045No Hit
ATCGATTCCTTGGTTCTGTGGAAAGGACGAAACACCGAACCCCTACCAAC10.40650406504065045No Hit
TAAGTAGAGTCTTGTGGAAAGGNCGAANCACCNNNNCCNNACCANNTGNN10.40650406504065045No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAATCACCGAACCCCTACC10.40650406504065045No Hit
CGATCGATACAGGTATTCTTGTGAAAGGACGAAACACCGAACCCCTACCA10.40650406504065045No Hit
TAACAATGGTCTTGTGGAAGGGAGGAACCCCGAACCCCATCCAAAATGGG10.40650406504065045No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAACACCGAACCCCTACC10.40650406504065045No Hit
TCGATCGTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT10.40650406504065045No Hit
ACGATCGATAAGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC10.40650406504065045No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCATACCA10.40650406504065045No Hit
TCGATCGTTACCATTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT10.40650406504065045No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAACT10.40650406504065045No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCA10.40650406504065045No Hit
TAAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG10.40650406504065045No Hit
TAACAATGGTCTTGTGGAAAGGACGAACACCGAACCCCTACCAACTGGTC10.40650406504065045No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)