FastQCFastQC Report
Tue 16 May 2023
H33GJBGXT_n01_R21h14d14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33GJBGXT_n01_R21h14d14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23579204
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC324557013.764544384110678No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA304659812.920699104176714No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA304473912.912815038200613No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT252845010.723220342807162No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC246300310.445657961990575No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT20649678.757577227797851No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC14964836.346622218459961No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT7511833.1857860850603776No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG4417821.8736086256346909No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG4269491.8107014978113765No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGATACACCGAACCCCTAC3766731.5974797113592132No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA2129360.9030669567980327No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGGT2097450.8895338451628817No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAATCACCGAACCCCTACC1345650.5706935653977123No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAATCACCGATCCCCTACC1273230.5399800603955927No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA1007330.42721119847811656No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGATCCCCTACC932000.3952635551225563No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGGT806910.3422125700256887No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC733290.31099014199122244No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC650680.27595503223942586No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTTCCAACTGG550670.23354053851860307No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGATCCCCTACC338200.1434314746163611No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTGGT271450.11512263094207931No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)