FastQCFastQC Report
Tue 16 May 2023
H33GJBGXT_n01_R21_h14d14rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33GJBGXT_n01_R21_h14d14rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA318.75No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA212.5No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC212.5No Hit
TCGATCCTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC16.25No Hit
AAAGTAGAGACTTTAGTAAAGGACGAAACACCGAACCCCTACCAACTGCA16.25No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT16.25No Hit
AAACAATGGTCTTGTGGAAAAAACGAAACACCGAACACCACCAAACACGT16.25No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC16.25No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT16.25No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCGGCATCCG16.25No Hit
ATCGATTCCTTGGTTCTTGTGCAAAGGACGAAACACCGAAACCCTACCAA16.25No Hit
TAAGAAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT16.25No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)