FastQCFastQC Report
Tue 16 May 2023
H33GJBGXT_n01_R19h0d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33GJBGXT_n01_R19h0d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25986752
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC287351311.057607353162103No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA277210310.667370050708914No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA25980739.99768266538273No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT22996008.849124353824594No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC22809418.777322383343636No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT22486328.652993648455952No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC19805977.621564249352901No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT18944067.289891403127255No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG13347835.136397961545945No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG12753534.90770451035974No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGATACACCGAACCCCTAC3343071.286451650440963No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGGT2529070.9732151213048865No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA2498230.9613475358521142No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGGT2002610.7706272796230941No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTTCCAACTGG1749530.6732391951098775No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAATCACCGAACCCCTACC1124190.43260119617872983No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA939050.3613572023160109No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAATCACCGATCCCCTACC831450.31995148912799876No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGATCCCCTACC818440.3149450920222735No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC575630.2215090212120391No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC569650.21920784867612542No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTGGT508700.1957535901370052No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTGGT272390.10481879382232917No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)