FastQCFastQC Report
Tue 16 May 2023
H33GJBGXT_n01_R17h7d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33GJBGXT_n01_R17h7d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21711047
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC269010012.390466475430689No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA261734712.055369784792045No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA260373011.992650561716346No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC21683079.987113933289352No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT21633279.964176301585088No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC18411828.48039249327773No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT17897538.243513083454705No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT9973474.5937305556936066No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG5995622.7615526786893327No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG5722032.6355384887702558No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGATACACCGAACCCCTAC3104771.4300415820572816No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA1889800.8704324577253229No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGGT1867730.8602671257632116No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAATCACCGAACCCCTACC1176990.5421157256948502No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGGT1107980.5103300637689191No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAATCACCGATCCCCTACC933150.42980423744649443No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGATCCCCTACC853930.39331590042617476No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA848690.39090238255207127No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTTCCAACTGG773570.3563024850897334No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC604300.2783375670459375No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC519200.2391409313424636No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTGGT250340.115305355840278No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATTCACCGATCCCCTACC222880.102657416751942No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTGGT221410.10198034208115343No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)