FastQCFastQC Report
Tue 16 May 2023
H33GJBGXT_n01_R15d14.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33GJBGXT_n01_R15d14.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2850209
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT109075538.26929884790905No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG67263723.59956761065592No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG62820122.0405240457805No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGGT1181994.147029217857357No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTTCCAACTGG854932.9995344201074374No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTGGT228090.8002571039527278No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTGGT69770.24478906634566097No Hit
TAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGTC34320.12041222240193614No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACAGGT34040.11942983830308584No Hit
TAAGTAGAGTCTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGTC31750.11139533978034592No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTTCCAACTGG29860.10476424711310645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)