FastQCFastQC Report
Tue 16 May 2023
H33GJBGXT_n01_R14d7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33GJBGXT_n01_R14d7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2997306
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT113253237.78499759450653No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG67547322.5360039982571No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG64810421.622884016513495No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGGT1350794.5066803322717135No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTTCCAACTGG918323.063817975208404No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTGGT284860.9503867806623681No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTGGT84420.2816529243260448No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA65890.21983074133905578No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA64370.21475952071626989No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC63290.21115628501060618No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT53740.17929433965033933No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC47290.15777501529707008No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC46370.15470559228854178No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT42300.14112673180516103No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTTCCAACTGG37270.12434499513896813No Hit
TAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGTC35390.11807269594762765No Hit
TAAGTAGAGTCTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGTC34300.11443609694839299No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACAGGT34000.1134351981412642No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)