FastQCFastQC Report
Tue 16 May 2023
H33GJBGXT_n01_R13d0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33GJBGXT_n01_R13d0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4691495
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT180213138.41272344956138No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG109014623.236644182717875No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG102076221.757712626785278No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGGT2055034.380330790078642No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTTCCAACTGG1427513.0427614225316235No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGATCCCCTAGCAACTGGT415780.886242018802109No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTAGCAACTGGT123860.26400966003374193No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACAGGT56890.12126198578491505No Hit
TAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGTC55830.11900257806946399No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGTACCCCTTCCAACTGG55550.11840575338991088No Hit
TAAGTAGAGTCTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGTC51420.10960258936650256No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)