FastQCFastQC Report
Tue 16 May 2023
H33GJBGXT_n01_R13_d0rc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH33GJBGXT_n01_R13_d0rc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3658
Sequences flagged as poor quality0
Sequence length151
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC59216.183706943685074No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA58015.855658829961728No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA51914.188080918534718No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC38610.552214324767633No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC3399.267359212684527No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT2907.927829414980864No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT2165.90486604702023No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT381.038819026790596No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGATACACCGAACCCCTAC350.9568069983597595No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA240.6560962274466922No Hit
ATACACGATCTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGG190.519409513395298No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGATCCCCTACC160.4373974849644614No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAATCACCGAACCCCTACC150.4100601421541826No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGGT110.30071077091306725No Hit
GATCGCGCGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTG100.2733734281027884No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAATCACCGAACCCCTACCA100.2733734281027884No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGATACACCGAACCCCTACC80.2186987424822307No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAATCACCGATCCCCTACC70.19136139967195187No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC70.19136139967195187No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCACTACCAA60.16402405686167304No Hit
TAAGTAGAGTCTTGTGGAAAGGACGAAACACCGATCCCCTACCAACTGGT50.1366867140513942No Hit
TAACAATGGTCTTGTGGAAAGGACGAAACACCGAACCCCAACCAACAGGT50.1366867140513942No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCAACCAA40.10934937124111535No Hit
ACGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC40.10934937124111535No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTATCAA40.10934937124111535No Hit
TAACTAGAGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACTGGT40.10934937124111535No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)