FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146793
Sequences flagged as poor quality0
Sequence length75
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG8036354.74579850537832No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGGGA150.002351116969.0023915
GCCAATC150.002351116969.0023913
GTACAGG200.007335392351.7694471
CCTAGGT200.007345297451.75179312
GGGCCCG200.007345297451.75179332
TACTTAC200.007355212251.7341467
CATCCGG200.007355212251.7341462
GTGCAGA350.001341496739.443391
TTGGCTG350.001343749739.42993550
TTTTCCC400.002588544334.50119417
TCCACTG400.002592879634.4894335
TCGGCCT450.004608902630.66772843
TCAGCCT500.007711984727.60095434
AGGTGTA1055.9872883E-419.71496869
GCCCGCG900.005709944819.1673334
GAGGTGT1702.4022065E-618.26533769
AGAGGTG1855.3801887E-616.78436568
GAGAGGT2353.170362E-614.68135969
GAATAGA4950.014.6368769
AGAGAGG2557.386134E-613.5298868
Produced by FastQC (version 0.11.2)