FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs94.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs94.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1448092
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG181221.2514398256464367No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38970.2691127359311425No Hit
GTATAGGGCCTGACGCCTGCCCGGTGCCTGAAGGTTAAGTGGAGGGGTGCAAGCTCTGAAATGAAGCCCAGGTAA14840.10247967670562368No Hit
GTATAGACCGTACCCGAAACCGACACAGGTGGTCAGGTAGAGTATACCAAGGCGCTTGAGAGAAGTGTCCTGAAG14690.1014438309168202No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA4900.027.4666631
CGATCTG87350.026.6167075
ATCTGGG11550.026.2819757
CACTATA2950.025.72515539
GATCTGG32400.025.2325236
ATCTGTG6450.025.1359627
ATCTGGC10650.024.6162197
ACTATAT3250.024.4085440
TTCCGAT233700.023.6051672
GACACTA4250.023.53782837
ATCTAGC5850.022.9967297
CTACCGT902.1287742E-422.9967299
TCCGATC240500.022.8628623
CCGATCT239050.022.856454
TAGGTGT17750.022.36206869
TAGTGCG1253.508594E-622.0768610
ATCTGAG7150.021.71027
ACACTAT4000.021.55943538
CTATATA4500.021.46064841
ACGTATT2750.021.32939720
Produced by FastQC (version 0.11.2)