FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1781719
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG184511.0355729494942805No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA65650.36846438748197663No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA5550.028.6035671
CGATCTG89350.026.2906075
GATCTGG32500.026.003446
ATCTGGG11550.025.6841097
CTTGTGT19450.025.553369
ATCTGGC11000.024.4596357
TTCCGAT240000.023.9358372
TCCGATC245950.023.424953
ATCTGTG5400.022.9962397
CCGATCT252200.022.7318084
CTTCCGA275450.021.1487661
TGTGTAG12650.021.00895169
ACACTAT3050.020.36018438
ATCTGAG7900.020.0853217
CGAGTAT2655.456968E-1219.52839726
TTGTGTA13800.019.50173868
TAAGTTC7800.019.45835730
CGATCTA48550.018.9701215
ACTAGAC1108.234214E-418.81985966
GTCTTAC7800.018.5827541
Produced by FastQC (version 0.11.2)