FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs92.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs92.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2389233
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG266511.1154625773208389No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA86110.36040854952195955No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA7750.028.9454731
ACACTAT7000.025.13543938
CACTATA6750.025.0441739
ATCTGAG8350.022.3087987
GATCTGG47100.022.264946
CGATCTG149300.022.2502755
CTATATA10000.022.07606141
ATCTGGC16350.021.0984787
ACTATAT9050.020.58196340
TCTGTGC6250.020.421648
ATCTGTG9650.020.3758837
AGTGTGT30900.020.3265569
GATCTTG21200.020.1769126
GACACTA9250.020.1403237
CCGATCT452650.019.0446344
TTCCGAT465400.018.6968942
TGTGTAG19350.018.5482669
TCCGATC470400.018.4958343
TAGGGAG35250.018.3000134
GATCTTT38850.018.1137356
Produced by FastQC (version 0.11.2)