FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1003571
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG158461.5789615283821474No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA151691.5115024248408933No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16880.168199360085136No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12550.12505343418651993No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGTG1052.3355824E-829.5645057
CGTGTAG6700.028.85208569
TTTCGGA4300.028.093031
ATCTGTG2350.027.8870877
GCTAGCA951.0472228E-525.43158358
CACTATA2050.025.24302739
CGATCTG52600.024.987435
CCTAATG851.4415068E-424.3569552
CTACGCT851.4454147E-424.347249
GTACAGG2600.023.894511
TACGTGT14900.023.16742369
TACTAGT750.001990937223.003792
ACTATTC2400.022.99461611
GGCTAGG1704.449248E-922.3183046
CAGGGGT5750.022.1981224
GATCTTT30250.022.00641611
GGCATAC953.0649285E-421.78980427
GATCTGG17700.021.6305276
CCGATCT167650.021.5645354
CGAAGTT800.002903118321.55745141
Produced by FastQC (version 0.11.2)