FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs90.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs90.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1350967
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53220.393940044427436No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15990.11835966385559381No Hit
GTCCTGGAGACAGTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCT14920.11043941117732706No Hit
GTATAGACCGTACCCGAAACCGACACAGGTGGTCAGGTAGAGTATACCAAGGCGCTTGAGAGAAGTGTCCTGAAG14850.10992126380585167No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAGC3550.030.1242547
CGATCTG41300.029.1513275
TTCCGAT104300.028.582012
ATAGTGT6100.027.72312769
TCCGATC108700.027.6144893
CGATCTA20400.027.2256765
GATCTCA9000.026.44786
ATCTGTG3700.026.1059387
TCTGTCC2000.025.8728478
CCGATCT120000.025.355394
GATCTAG8500.025.1626136
ATCTAGT2100.024.6408087
CTTCCGA124950.024.1933271
GATCTGC13600.024.097266
TCTAGCC2350.023.487418
GATGCAT1052.2767543E-523.00575461
TCTAGGG1502.8849172E-823.0023442
AGTTCCG12350.022.3463232
ACGAATG5750.021.59660145
TAGTGTA2900.021.4215369
Produced by FastQC (version 0.11.2)