FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2443555
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG433161.7726631894923586No Hit
GGTAGGGATACCCGCTGAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACG36610.14982269684946728No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32490.13296201640642424No Hit
CTCTACTTGTGCGCTATCGGTCTCCCGCCAATATTTAGCTTTAGATGGAATTTACCACCCATTTTGAGCTGCATT28010.11462807262369785No Hit
GAACTTAAGCATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTCAGTAACGGCGAGTGAAGCGGCAA26140.1069752880536759No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTACT14450.033.1941381
CTACTTG14350.032.456263
GCGCTAT14650.032.26393511
TACTTGT14750.031.8093414
TCTACTT15200.031.3241162
TGCGCTA15050.030.94793510
CGCTATC15400.030.0137812
GCTATCG15600.029.18796213
TTGTGCG17100.028.0442777
TGTGCGC17100.027.6407628
TTTCGGA5600.027.1130941
ACTTGTG17350.026.8447785
TATCGGT18000.025.68161215
GATCTGG30100.024.8724616
ATCGGTC18050.024.65485616
TCTAGTG1401.3893441E-824.6432238
GCCAATA17100.024.21237227
GTGCGCT19150.024.141359
CGCCAAT17400.023.99320826
GGTCTCC18500.023.50005119
Produced by FastQC (version 0.11.2)