FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs88.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs88.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126062
Sequences flagged as poor quality0
Sequence length75
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47793242.442778461576715No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA2150.038.5215261
TATAGGA450.00462286930.6663682
CACTATA1800.028.74972239
ACACTAT2100.027.92830338
ACATTAC500.007735214727.5997333
TCTAGTG756.169401E-527.5960588
GACCTAG500.00774024727.5960587
ACTATAT2400.027.30617140
GACACTA2450.026.75484337
TACAGTA658.657471E-426.53820668
CGATCTG36600.025.1655525
GATCTGG12950.025.0388936
TAGAATC700.001333904124.6426266
CTATATA2850.024.20491641
AACTATT750.001990534823.00488957
GGGACAC4500.022.99977935
ATCTAGT1502.8912837E-822.9967147
ATCTAGC2700.022.9967147
ATCTGGG4850.022.0483937
ATTATCA953.0685298E-421.786369
Produced by FastQC (version 0.11.2)