FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs84.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs84.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1702707
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG135020.7929726018627984No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA71980.4227386156279383No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA6800.034.0000881
ACACTAT5300.029.29025738
CACTATA5650.025.03352239
AGTGTGT25250.024.3296969
GACACTA6900.023.99820137
ATCTGTG8300.022.4434417
CGATCTG129850.021.705985
GACCTAT800.002901754621.56025136
ATCTGGC13450.021.544227
ACTATAT7150.021.22730640
ACCTAGC1004.3390394E-420.697848
ATCTGCG9850.020.6628197
CTATATA8000.019.83368141
GATCTGG38000.019.608486
ATCTAGC7800.019.4595077
CTAGGCG1451.2665254E-519.0324979
GGGACAC11350.018.84440435
TCTAGGG5550.018.6488952
CCGATCT369600.018.5186374
TGTGTAG15650.018.30381869
Produced by FastQC (version 0.11.2)