FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs83.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs83.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1433226
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG174511.2176028065357452No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA107420.7494979856631124No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA5050.031.4340271
GTCTACG1759.094947E-1225.6353281
CACTATA3350.024.7160339
GATCTGG24200.024.6611166
TACGTGT19150.024.148269
ACCTAGG1202.4529163E-623.0060651
TAGGCGT750.001993847322.9988374
ACACTAT3650.022.68457438
CGATCTG90550.021.9821075
ATCTGGC10550.021.5810957
GACACTA4000.021.56216237
ATCTGGG9550.021.3123137
CCTAGGG4100.021.036992
TTACGTG22700.020.2133468
TCGCGTA1206.385824E-520.1260928
CCGATCT259500.018.9574224
CTATATA4750.018.8799641
CTCTAGG1301.178899E-418.5818211
CGATCTT59800.018.4599595
TTCCGAT267100.018.3934632
Produced by FastQC (version 0.11.2)