FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs82.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs82.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010204
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG113341.1219516058142711No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32600.32270709678441184No Hit
GTCCTGGAGACAGTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCT12140.12017374708474723No Hit
GTATAGACCGTACCCGAAACCGACACAGGTGGTCAGGTAGAGTATACCAAGGCGCTTGAGAGAAGTGTCCTGAAG11110.10997778666487165No Hit
GTCCTGAAGGAACTCGGCAAATTGCCTCCGTACCTTCGGAAGAAGGAGGCCCTCATTGTGCGCAAGCATTTTGAG10690.10582021057133015No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTGT9050.027.83742169
AATTCGC658.653099E-426.54017623
TAATGTG700.001334772624.6395685
ATCTGGC7250.023.3141297
ACTATTC1202.459139E-622.9980711
CGATCTG52150.022.4898055
GATCTGG17100.021.9882936
GATCTTG9500.021.7865666
AGGACTA1455.298443E-721.4109345
TTTCGGA3250.021.2363471
TGGACAG3250.021.2279365
TACTATG1154.6031448E-520.9992752
CAGTATT850.004113577320.299451
GAACGTA2051.7407729E-920.19442618
CCGATCT165200.020.0875134
ATCTTTT12950.019.9770412
TTCCGAT167100.019.9230122
TAGGACC9350.019.9224744
CTAGGAC1751.4743637E-719.7116553
TCCGATC169000.019.6970753
Produced by FastQC (version 0.11.2)