FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483568
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG284081.9148431349287662No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36020.24279305026800257No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA4700.028.6355251
ACGGTGT15600.024.55896269
CACTATA4750.023.96611239
ACACTAT5500.023.20685638
ATCTGGC13200.021.9531737
GACACTA6000.021.27295137
ATTCTAA3450.021.005771
TCTGGCC6600.020.9077848
ACTATAT6550.020.53723740
CTATATA6100.020.35595141
GTATTGT1751.4744364E-719.713056
CCGTAGT2303.45608E-1019.50009518
GTAAGTT7400.019.11624729
AGTTCCG7050.019.08133932
ACCGCGT3450.018.9988128
TTGTCGG7100.018.95271322
GATCTGG30800.018.929016
GGGACAC10050.018.8787835
TCAATTG1108.234257E-418.81954229
GGTGTAG11250.018.71496269
Produced by FastQC (version 0.11.2)