FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1325617
Sequences flagged as poor quality0
Sequence length75
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG176381.3305502268000484No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49070.3701672504199931No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGGA4550.034.8910451
ATCTGGC10750.026.6372687
ACACTAT2800.025.87310638
GATCTGG28450.025.3445196
CGATCTG92650.025.0977345
GACTTAT1651.1459633E-1025.09096568
TCTTAGC1551.5261321E-924.4839238
CTATAGG750.001989224523.0078621
TCTAGTG1653.1450327E-923.0000518
ATCTGTG5750.022.2000487
ATCTGGG11600.022.0086697
TAGGTGT18700.021.95956469
TACCTAG1004.3341203E-420.70082729
GAACCTA1004.3341203E-420.70082727
ATCTTAG2501.8189894E-1220.7000477
GACACTA5050.020.49354737
TCTGTCC5900.020.4661458
CCGATCT285850.020.4453834
ATCTAGC6250.020.4240447
TTCCGAT289800.020.2968982
Produced by FastQC (version 0.11.2)