FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1483560
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108780.733236269513872No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA29560.19925045161638222No Hit
GTCCTGGAGACAGTGGGGAAGTCGTTACGCCATTCGTGCAGGTCGGAACTTACCCGACAAGGAATTTCGCTACCT17250.11627436706301059No Hit
GTATAGGGCCTGACGCCTGCCCGGTGCCTGAAGGTTAAGTGGAGGGGTGCAAGCTCTGAAATGAAGCCCAGGTAA17170.11573512362155894No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTGTG5650.026.8638977
CTTGTGT14250.026.3959669
TTTCGGA4800.025.8865971
CGATCTG88650.025.4511685
ATCTGGG11100.024.2402117
GATCTGG30200.024.1012926
ATCTGAG8650.023.9276437
CTAGGGG3500.023.658973
TTGTGTA9200.023.63080869
TTCCGAT232050.023.017422
CACTATA3450.022.99867239
GCAACTA2600.022.55715427
ATCTGGC11600.022.3032447
CCGATCT239450.022.2454474
TCCGATC243950.022.0494583
ACACTAT3300.021.9532838
GTTCGTA1756.206392E-921.68519426
CGATCTA47700.021.6252695
TAGGACT3650.020.7940224
ACTATAT4150.020.78193540
Produced by FastQC (version 0.11.2)