FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537175
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40670.26457625189064354No Hit
GTTCTATCCCCATCACGACAGAGTTTCACAAGATTACCCATGCCTTCCGGCAAAGGTGGTAGACTCGCTGGCTCT18420.11983020801144958No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATAGG601.3416926E-534.5043141
CTAGGCG400.002597086434.49879
ATCTATG2250.029.1322337
TTTATAC500.00773714227.5989573
TCTATGC1404.6929927E-1027.1061218
ATCTGCG4600.026.2490087
TCCTAGG1251.2635792E-724.8431031
CCGATCT148700.024.4290164
CTTCCGA151500.024.3010581
TTCCGAT151350.024.1404042
TCCGATC152750.023.9401783
CCTAGGG2950.023.390472
ATCTGAG5050.023.2268477
ACCTAGG1052.2792226E-523.0028741
CGATCTG61950.022.9434455
CGATCTA32750.022.4373235
CGATCTT29500.022.21955
ATCTGGG8550.022.1921467
GATCTTT10400.021.5616876
TGTGTAG4500.021.47703769
Produced by FastQC (version 0.11.2)