FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1469481
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG119140.8107624392557645No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27540.1874131070765801No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGTGT11250.027.61126569
TTTCGGA4450.026.3613341
CTCTAGG951.050476E-525.422771
CGATCTG80400.025.2304735
ATCTGAG6650.023.8638367
GATCTGG27000.022.360346
CCGATCT217800.022.2064654
TTCCGAT220900.022.09712
TCCGATC222800.021.9094053
ACACTAT3000.021.84775738
ATCTGGC11300.021.6762437
ATCTTTT17250.021.59484312
ATCTGTG5450.020.8891877
ATCTAGC6300.020.8088057
TCTAGGG2402.910383E-1120.1229342
CTTCCGA249500.019.9269981
GATCTAG12850.019.5985456
TATGCTT2655.456968E-1219.5276285
CGATCTA41300.019.4630095
TCTGACC5150.019.4265148
Produced by FastQC (version 0.11.2)