FastQCFastQC Report
Sun 20 May 2018
H32MNAFXY_n02_zt2wtlarvs71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH32MNAFXY_n02_zt2wtlarvs71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences667485
Sequences flagged as poor quality0
Sequence length75
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46330.6940979947114917No Hit
ATCTAGGACTACCATGGTTGCAACGGGTAACGGGGAATCAGGGTTCGATTCCGGAGAGGGAGCCTGAGAAACGGC10720.16060285998936308No Hit
TCGTAAGACTAACTTAAGCGAAAGCATTTGCCAAAGATGTTTTCATTAATCAAGAACGAAAGTTAGAGGTTCGAA6990.10472145441470596No Hit
GTCCTAGATACTACCATCAAAAGTTGATAGGGCAGACATTTGAAAGATCTGTCGTCGGTACAAGACCATACGATC6890.10322329340734249No Hit
CCTTAGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAATCGAACCCTGATTCCCCGTTACCCGTTGCAACCA6690.10022697139261556No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGTGT4150.031.59441269
TCTATGT901.9934487E-730.66600227
CACTATA1700.030.44051639
ACTATAT2100.027.92796540
CTCTAGG756.1449005E-527.6097451
ACACTAT1900.027.2362538
CTATATA2450.026.7545241
TTTCGGA1951.8189894E-1224.7779771
GATCTGG8850.023.7773656
GGGACAC3050.023.75358435
AGCCCGT2350.023.48709310
ACCGTTA2800.023.4084538
ATCTGGG4300.023.2651947
GCACTTA2400.023.00122523
ATTATGG1202.455734E-622.99953
CTTGCCT750.00199327122.9977789
TATGACA2600.022.5555114
GCCCGTT2450.022.52843511
GGCACTT2650.022.13325122
GGTAAGT2207.2759576E-1221.9540728
Produced by FastQC (version 0.11.2)